Abstract
An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time- and concentration-dependent production of mCherry could be correlated to TetR-controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.
© 2011 The Authors. Microbial Biotechnology © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Gene Expression
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Gene Expression Regulation, Bacterial*
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Genes, Reporter
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism*
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Peptides / genetics
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Peptides / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Staphylococcus aureus / genetics
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Staphylococcus aureus / metabolism*
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Tetracycline / metabolism
Substances
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Bacterial Proteins
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Luminescent Proteins
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Peptides
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Recombinant Fusion Proteins
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Tetracycline