Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence

Microb Biotechnol. 2012 Jan;5(1):129-34. doi: 10.1111/j.1751-7915.2011.00304.x. Epub 2011 Sep 29.

Abstract

An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time- and concentration-dependent production of mCherry could be correlated to TetR-controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Gene Expression
  • Gene Expression Regulation, Bacterial*
  • Genes, Reporter
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism*
  • Peptides / genetics
  • Peptides / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / metabolism*
  • Tetracycline / metabolism

Substances

  • Bacterial Proteins
  • Luminescent Proteins
  • Peptides
  • Recombinant Fusion Proteins
  • Tetracycline