Optical sectioning microscopy with planar or structured illumination

Nat Methods. 2011 Sep 29;8(10):811-9. doi: 10.1038/nmeth.1709.

Abstract

A key requirement for performing three-dimensional (3D) imaging using optical microscopes is that they be capable of optical sectioning by distinguishing in-focus signal from out-of-focus background. Common techniques for fluorescence optical sectioning are confocal laser scanning microscopy and two-photon microscopy. But there is increasing interest in alternative optical sectioning techniques, particularly for applications involving high speeds, large fields of view or long-term imaging. In this Review, I examine two such techniques, based on planar illumination or structured illumination. The goal is to describe the advantages and disadvantages of these techniques.

Publication types

  • Review

MeSH terms

  • Animals
  • Imaging, Three-Dimensional / instrumentation
  • Imaging, Three-Dimensional / methods*
  • Microscopy, Confocal / methods*
  • Microscopy, Fluorescence / methods*
  • Optics and Photonics / instrumentation
  • Optics and Photonics / methods*