Protective effect of hydrogen-rich water against gentamicin-induced nephrotoxicity in rats using blood oxygenation level-dependent MR imaging

Magn Reson Med Sci. 2011;10(3):169-76. doi: 10.2463/mrms.10.169.


Purpose: We assessed intrarenal oxygenation in gentamicin-induced nephrotoxicity (GIN) and the protective effect of hydrogen-rich water (HW) against GIN using blood oxygenation level-dependent magnetic resonance (MR) imaging.

Materials and methods: We acquired T(2)*-weighted images (T(2)*WI) of 21 rats on Days 0, 2, 4, and 7 using a 1.5-tesla MR imaging system. The rats were divided into 3 groups of seven each: control rats had free access to standard water and no gentamicin (GM) injection; rats designated the GM group had free access to standard water and were injected with GM (80 mg/kg/day) subcutaneously for 7 days; and the third group, designated the GM+HW group, had free access to HW and were injected with GM. R(2)* (=1/T(2)*) was estimated from T(2)*WI.

Results: R(2)* values in the cortex were significantly decreased on Days 2, 4, and 7 compared with those on Day 0 in the GM group but not significantly changed in the control and GM+HW groups. R(2)* values in the medulla did not change significantly in any group.

Conclusions: Our findings suggested reduced oxygen utility, mainly in the cortex, in gentamicin-induced nephrotoxicity and an ameliorative effect of hydrogen-rich water against GIN.

MeSH terms

  • Analysis of Variance
  • Animals
  • Blood Urea Nitrogen
  • Creatinine / blood
  • Gentamicins / toxicity*
  • Hydrogen / pharmacology*
  • Kidney Diseases / chemically induced*
  • Kidney Diseases / prevention & control*
  • Magnetic Resonance Imaging / methods*
  • Male
  • Oxygen / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Water / pharmacology*


  • Gentamicins
  • Water
  • Hydrogen
  • Creatinine
  • Oxygen