Abstract
A DNA strand containing a triazole phosphodiester mimic is an efficient template for in vitro transcription. This is the first demonstration of transcription through a heavily modified DNA backbone linkage and it suggests that click-ligated DNA could be useful for the direct synthesis of biologically active RNA and proteins.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Click Chemistry
-
DNA / chemistry*
-
DNA-Directed RNA Polymerases / chemistry
-
RNA / chemical synthesis*
-
Transcription, Genetic
-
Triazoles / chemistry*
-
Viral Proteins / chemistry
Substances
-
Triazoles
-
Viral Proteins
-
RNA
-
DNA
-
bacteriophage T7 RNA polymerase
-
DNA-Directed RNA Polymerases