Sleep loss alters synaptic and intrinsic neuronal properties in mouse prefrontal cortex

Brain Res. 2011 Oct 28:1420:1-7. doi: 10.1016/j.brainres.2011.08.078. Epub 2011 Sep 7.


Despite sleep-loss-induced cognitive deficits, little is known about the cellular adaptations that occur with sleep loss. We used brain slices obtained from mice that were sleep deprived for 8h to examine the electrophysiological effects of sleep deprivation (SD). We employed a modified pedestal (flowerpot) over water method for SD that eliminated rapid eye movement sleep and greatly reduced non-rapid eye movement sleep. In layer V/VI pyramidal cells of the medial prefrontal cortex, miniature excitatory post synaptic current amplitude was slightly reduced, miniature inhibitory post synaptic currents were unaffected, and intrinsic membrane excitability was increased after SD.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / drug effects
  • Action Potentials / physiology
  • Analysis of Variance
  • Animals
  • Electric Stimulation
  • Electroencephalography
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • Eye Movements
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Patch-Clamp Techniques
  • Prefrontal Cortex / pathology*
  • Pyramidal Cells / drug effects
  • Pyramidal Cells / physiopathology*
  • Sleep Deprivation / pathology*
  • Sleep Deprivation / physiopathology
  • Time Factors