Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging

Nat Methods. 2011 Oct 2;8(11):933-5. doi: 10.1038/nmeth.1716.


We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables large-scale, multiplexed quantitative proteomics using isobaric tagging.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Gases*
  • Humans
  • Proteins / chemistry*
  • Proteome*
  • Tandem Mass Spectrometry / methods


  • Gases
  • Proteins
  • Proteome