Trimethyltin chloride (TMT) neurobehavioral toxicity in embryonic zebrafish

Neurotoxicol Teratol. Nov-Dec 2011;33(6):721-6. doi: 10.1016/j.ntt.2011.09.003. Epub 2011 Sep 19.

Abstract

Trimethyltin chloride (TMT) is a neurotoxicant that is widely present in the aquatic environment, primarily from the manufacture of PVC plastic, but few studies have evaluated aquatic neurotoxicity. We have examined TMT dose-dependent malformation and neurobehavioral toxicity in the embryonic zebrafish model. Exposure of embryos to TMT (0-10 μM) from 48 to 72 hours post fertilization (hpf) elicited a concentration-related increase (0-100%) in malformation incidence with an EC(25) of 5.55 μM. TMT also significantly modulated the frequency of tail flexion, the earliest motor behavior observed in developing zebrafish, and the ability to respond to a mechanical tail touch. Exposure to 5 μM TMT from 48 to 72 hpf modulated the photomotor response at 4 and 5 days post fertilization and significantly promoted apoptosis in the tail. Our study demonstrates the morphological and behavioral sensitivity of the developing zebrafish to TMT and establishes a platform for future identification of the affected pathways and chemical modulators of TMT toxicity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Behavior, Animal / drug effects*
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Embryo, Nonmammalian / abnormalities
  • Embryo, Nonmammalian / drug effects*
  • Embryo, Nonmammalian / pathology
  • Embryonic Development / drug effects
  • Neurotoxicity Syndromes / embryology*
  • Neurotoxicity Syndromes / pathology
  • Neurotoxicity Syndromes / physiopathology
  • Tail / abnormalities
  • Tail / drug effects
  • Tail / embryology
  • Tail / pathology
  • Trimethyltin Compounds / toxicity*
  • Water Pollutants, Chemical / toxicity*
  • Zebrafish / abnormalities
  • Zebrafish / embryology*

Substances

  • Trimethyltin Compounds
  • Water Pollutants, Chemical
  • trimethyltin chloride