MALDI-TOF MS improves routine identification of non-fermenting Gram negative isolates from cystic fibrosis patients

J Cyst Fibros. 2012 Jan;11(1):59-62. doi: 10.1016/j.jcf.2011.09.001. Epub 2011 Oct 2.


Identification of non-fermenting Gram-negative bacteria (NFGNB) from cystic fibrosis (CF) patients is often limited. A collection of stored NFGNB isolates (n=182) recovered from CF patients over a 15 year period was examined. The routinely reported identification during this period was compared with that obtained by MALDI-TOF MS. Isolates giving discrepant identification at the genus level were further analyzed by 16S rDNA sequencing. The MALDI-TOF MS system identified 94% of the isolates, including Burkholderia cepacia and Pandoraea spp. isolates, the latter previously misidentified as other NFGNB by conventional microbiological methods. Lack of identification by MALDI-TOF MS was associated with the absence of entries in the database.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Achromobacter / isolation & purification
  • Actinobacteria / isolation & purification
  • Burkholderia cepacia / isolation & purification
  • Chryseobacterium / isolation & purification
  • Cystic Fibrosis / microbiology*
  • Gram-Negative Aerobic Bacteria / isolation & purification*
  • Humans
  • Proteomics
  • Pseudomonas / isolation & purification
  • Ralstonia pickettii / isolation & purification
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*