Reliable protein production in a Pseudomonas fluorescens expression system

Protein Expr Purif. 2012 Feb;81(2):157-65. doi: 10.1016/j.pep.2011.09.010. Epub 2011 Sep 24.

Abstract

A bottleneck to product development can be reliable expression of active target protein. A wide array of recombinant proteins in development, including an ever growing number of non-natural proteins, is being expressed in a variety of expression systems. A Pseudomonas fluorescens expression platform has been developed specifically for recombinant protein production. The development of an integrated molecular toolbox of expression elements and host strains, along with automation of strain screening is described. Examples of strain screening and scale-up experiments show rapid development of expression strains producing a wide variety of proteins in a soluble active form.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Antibodies / genetics
  • Antibodies / metabolism
  • Antigens / genetics
  • Antigens / metabolism
  • Cloning, Molecular
  • Enzyme Activation
  • Gene Expression Regulation, Bacterial*
  • Genes, Bacterial
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism*
  • Plasmids / genetics
  • Plasmids / metabolism
  • Promoter Regions, Genetic
  • Protein Folding
  • Pseudomonas fluorescens / genetics
  • Pseudomonas fluorescens / metabolism*
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Solubility
  • Transcription, Genetic
  • Vaccines / genetics
  • Vaccines / metabolism

Substances

  • Antibodies
  • Antigens
  • Recombinant Proteins
  • Vaccines