A candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle

Cell. 1990 Jul 27;62(2):213-24. doi: 10.1016/0092-8674(90)90360-q.

Abstract

Probes derived from cDNAs encoding isozymes of rat protein kinase C (PKC) were used to screen the genome of the budding yeast S. cerevisiae. A single gene (PKC1) was isolated that encodes a putative protein kinase closely related to the alpha, beta, and gamma subspecies of mammalian PKC. Deletion of PKC1 resulted in recessive lethality. Cells depleted of the PKC1 gene product displayed a uniform phenotype, a characteristic of cell division cycle (cdc) mutants, and arrested cell division at a point subsequent to DNA replication, but prior to mitosis. Unlike most cdc mutants, which continue to grow in the absence of cell division, PKC1-depleted cells arrested growth with small buds. PKC1 may regulate a previously unrecognized checkpoint in the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle*
  • Chromosome Mapping
  • Gene Expression
  • Genes, Fungal*
  • Genetic Linkage
  • Molecular Sequence Data
  • Protein Kinase C / genetics*
  • RNA, Messenger / genetics
  • Recombination, Genetic
  • Restriction Mapping
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / genetics

Substances

  • RNA, Messenger
  • Protein Kinase C

Associated data

  • GENBANK/M32491