Abstract
We have identified a yeast gene, SFL2 (suppressor gene for flocculation), which complemented a newly isolated sfl2 mutant. This mutation causes asexual cell aggregation. The strain bearing the SFL2 gene disruption exhibited pleiotropic phenotypes characteristic for tup1 mutants. Physical mapping and complementation analysis suggested that the cloned SFL2 gene is identical to the TUP1 gene. The SFL2 gene encodes a 669-amino acid protein which has domains rich in glutamine, as does the SSN6 protein.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Genes, Fungal*
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Genotype
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Molecular Sequence Data
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Mutation*
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Nuclear Proteins*
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Oligonucleotide Probes
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Phenotype
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Repressor Proteins*
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Restriction Mapping
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins*
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Suppression, Genetic*
Substances
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Nuclear Proteins
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Oligonucleotide Probes
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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TUP1 protein, S cerevisiae