Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
, 51 (6), 707-18

Combined Fish Oil and Astaxanthin Supplementation Modulates Rat Lymphocyte Function

Affiliations
Comparative Study

Combined Fish Oil and Astaxanthin Supplementation Modulates Rat Lymphocyte Function

Rosemari Otton et al. Eur J Nutr.

Abstract

Purpose: Higher intakes of n-3 polyunsaturated fatty acids that are abundant in marine fishes have been long described as a "good nutritional intervention" with increasing clinical benefits to cardiovascular health, inflammation, mental, and neurodegenerative diseases. The present study was designed to investigate the effect of daily fish oil (FO-10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) intake by oral gavage associated with the antioxidant astaxanthin (ASTA-1 mg/kg BW) on the redox metabolism and the functional properties of lymphocytes from rat lymph nodes.

Methods: This study was conducted by measurements of lymphocyte proliferation capacity, ROS production [superoxide (O₂(•-)) and hydrogen peroxide (H₂O₂)], nitric oxide (NO(•)) generation, intracellular calcium release, oxidative damage to lipids and proteins, activities of major antioxidant enzymes, GSH/GSSG content, and cytokines release.

Results: After 45 days of FO + ASTA supplementation, the proliferation capacity of activated T- and B-lymphocytes was significantly diminished followed by lower levels of O₂(•-), H₂O₂ and NO(•) production, and increased activities of total/SOD, GR and GPx, and calcium release in cytosol. ASTA was able to prevent oxidative modification in cell structures through the suppression of the oxidative stress condition imposed by FO. L: -selectin was increased by FO, and IL-1β was decreased only by ASTA supplementation.

Conclusion: We can propose that association of ASTA with FO could be a good strategy to prevent oxidative stress induced by polyunsaturated fatty acids and also to potentiate immuno-modulatory effects of FO.

Similar articles

See all similar articles

Cited by 7 PubMed Central articles

See all "Cited by" articles

References

    1. J Clin Epidemiol. 1995 Nov;48(11):1379-90 - PubMed
    1. J Leukoc Biol. 2008 Nov;84(5):1223-37 - PubMed
    1. Eur J Pharm Sci. 2003 Dec;20(4-5):375-82 - PubMed
    1. Postgrad Med. 2009 Nov;121(6):148-57 - PubMed
    1. Nature. 2003 Oct 30;425(6961):980-4 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback