Live-cell 3D super-resolution imaging in thick biological samples

Nat Methods. 2011 Oct 9;8(12):1047-9. doi: 10.1038/nmeth.1744.


We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.

MeSH terms

  • Cell Line
  • Cell Survival
  • Humans
  • Imaging, Three-Dimensional / methods*
  • Microscopy, Fluorescence / methods*
  • Nanocapsules
  • Phantoms, Imaging
  • Spheroids, Cellular / cytology*


  • Nanocapsules