Can gap junctions deliver?

Biochim Biophys Acta. 2012 Aug;1818(8):2076-81. doi: 10.1016/j.bbamem.2011.09.025. Epub 2011 Oct 2.

Abstract

In vivo delivery of small interfering RNAs (siRNAs) to target cells via the extracellular space has been hampered by dilution effects and immune responses. Gap junction-mediated transfer between cells avoids the extracellular space and its associated limitations. Because of these advantages cell based delivery via gap junctions has emerged as a viable alternative for siRNA or miRNA delivery. Here we discuss the advantages and disadvantages of extracellular delivery and cell to cell delivery via gap junction channels composed of connexins. This article is part of a Special Issue entitled: The Communicating junctions, composition, structure and characteristics.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Biophysics / methods
  • Cell Communication
  • Cells, Cultured
  • Connexin 43 / metabolism
  • Connexins / metabolism*
  • Gap Junction alpha-5 Protein
  • Gap Junctions / metabolism
  • Gap Junctions / physiology*
  • Humans
  • Mesenchymal Stem Cells / cytology
  • Models, Biological
  • Pinocytosis
  • RNA, Small Interfering / metabolism*
  • RNA, Small Interfering / therapeutic use*
  • Rats

Substances

  • Connexin 43
  • Connexins
  • RNA, Small Interfering