Profile and quantification of glucosinolates in Pentadiplandra brazzeana Baillon

Phytochemistry. 2012 Jan;73(1):51-6. doi: 10.1016/j.phytochem.2011.09.006. Epub 2011 Oct 10.


Glucosinolates (GLs) present in root, seed, and leaf extracts of Pentadiplandra brazzeana Baillon were characterized and quantified according to the ISO 9167-1 method based on the HPLC analysis of desulfo-GLs. The analyses were complemented by GC-MS analyses of the isothiocyanates (ITCs) generated from GL degradation by myrosinase. Glucotropaeolin (1a), glucolimnanthin (2a), and glucoaubrietin (3a) were shown to be present in the root extract, whereas the seed mainly contained 3a. 3,4-Dimethoxybenzyl GL (4a), glucobrassicin (5a) and traces of 1a were detected in the leaf extract. The products were fully characterized as their desulfo-counterparts by spectroscopic techniques.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassicaceae / chemistry*
  • Cameroon
  • Chromatography, High Pressure Liquid
  • Glucosinolates / analysis*
  • Glucosinolates / chemistry
  • Glucosinolates / metabolism
  • Indoles / analysis
  • Indoles / chemistry*
  • Isothiocyanates / analysis
  • Isothiocyanates / chemistry*
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Plant Leaves / chemistry
  • Plant Roots / chemistry
  • Seeds / chemistry


  • Glucosinolates
  • Indoles
  • Isothiocyanates
  • glucotropeolin
  • glucobrassicin