Isolation of a novel protein kinase-encoding gene from yeast by oligodeoxyribonucleotide probing

Gene. 1990 May 31;90(1):87-92. doi: 10.1016/0378-1119(90)90442-t.

Abstract

We have identified a novel protein kinase-encoding gene, KIN3, in the genome of the budding yeast Saccharomyces cerevisiae. The gene was isolated from a library of cloned genomic fragments by probing with an oligodeoxyribonucleotide mixture corresponding to part of a highly-conserved region in the catalytic domain of protein serine-threonine kinases. KIN3 is unique in the yeast genome, maps to chromosome VI and is actively expressed in mitotically dividing cells to produce a 1400 nucleotide (nt) message. The nt sequence of KIN3 predicts a protein product of 43.4 kDa which contains all of the conserved elements found in known protein serine-threonine kinases, although the organisation of these elements in the KIN3 gene product differs significantly from the consensus. The function of the KIN3-encoded protein kinase is unclear although it appears not to be essential for growth, conjugation or sporulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Fungal Proteins / genetics*
  • Genes, Fungal*
  • Molecular Sequence Data
  • Molecular Weight
  • Oligonucleotide Probes
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*

Substances

  • Fungal Proteins
  • Oligonucleotide Probes
  • Saccharomyces cerevisiae Proteins
  • Protein Kinases
  • KIN3 protein, S cerevisiae
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/M55416