Tissue-Specific stem cell differentiation in an in vitro airway model

Macromol Biosci. 2011 Nov 10;11(11):1467-77. doi: 10.1002/mabi.201100181. Epub 2011 Oct 12.


The respiratory tract is the primary site of exposure to airborne compounds, with the bronchial epithelium providing one of the first lines of defence. A growing need exists for an accurate in vitro model of the bronchial epithelium. Here, normal human bronchial epithelial (NHBE) cells cultured at an air/liquid interface create a fully differentiated, in-vivo-like model of the human bronchial epithelium. Developmental characterisation includes (i) trans-epithelial electrical resistance, (ii) morphology and (iii) bronchial cell specific stains/markers. It is concluded that the basal/progenitor cells create a pseudo-stratified, mucociliary NHBE model containing basal, serous, Clara, goblet and ciliated cells, reflective of the normal human bronchial epithelium (days 24-33 ALI culture).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autopsy
  • Biomarkers / analysis
  • Bronchi / cytology*
  • Bronchi / metabolism
  • Cell Culture Techniques
  • Cell Differentiation
  • Cells, Cultured
  • Electric Impedance
  • Epithelial Cells / cytology*
  • Epithelial Cells / metabolism
  • Humans
  • Immunohistochemistry
  • Microscopy, Electron, Scanning
  • Respiratory Mucosa / cytology*
  • Respiratory Mucosa / metabolism
  • Tissue Engineering / methods*


  • Biomarkers