Trichoderma Reesei RUT-C30--thirty Years of Strain Improvement

Microbiology. 2012 Jan;158(Pt 1):58-68. doi: 10.1099/mic.0.054031-0. Epub 2011 Oct 13.


The hypersecreting mutant Trichoderma reesei RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic- and proteomic-based research on the RUT-C30 strain published over the last 30 years.

Publication types

  • Historical Article
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Fungal Proteins / genetics
  • Fungal Proteins / history
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Genetic Engineering / history
  • Genomics / history
  • History, 20th Century
  • History, 21st Century
  • Trichoderma / genetics*
  • Trichoderma / metabolism


  • Fungal Proteins