Updated multiplex polymerase chain reaction for detection of 16S rRNA methylases: high prevalence among NDM-1 producers

Diagn Microbiol Infect Dis. 2011 Dec;71(4):442-5. doi: 10.1016/j.diagmicrobio.2011.08.016. Epub 2011 Oct 13.

Abstract

We develop a rapid (<4 h) and reliable multiplex polymerase chain reaction for screening of 16S rRNA methylase genes conferring resistance to aminoglycosides. This study particularly underlined that 16S rRNA methylases are frequently (75%) identified among Enterobacteriaceae isolates producing the carbapenemase NDM-1.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides / pharmacology
  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Bacterial
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / genetics*
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Prevalence
  • Time Factors
  • tRNA Methyltransferases / genetics*

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • tRNA Methyltransferases