Feeding behaviors and ORXR-β-GABA A R subunit interactions in Carassius auratus

Neurotoxicol Teratol. Nov-Dec 2011;33(6):641-50. doi: 10.1016/j.ntt.2011.09.008. Epub 2011 Oct 5.

Abstract

Orexins are one of the most potent orexigenic factors in fish that through their interaction with the GABA(A) receptor system assures the successful execution of feeding, motor and sleep-wake activities. In the present study, the effects of ORX-A (10ng/g BW) very greatly enhanced (p<0.001) the time spent in feeding behaviors while at the same time moderately increased (p<0.05) food intake of the goldfish. It is worthy to note that the great variations of time spent in feeding behaviors induced by β GABA(A)R agonist (muscimol, MUS) and antagonist (bicuculline, BIC) did not result to be correlated to any significant variations of food intake. It was, however, a T-maze study allowing us to establish that learning and mnemonic events very likely also operated in an ORX-A+GABA(A)R-dependent fashion in our fish model. Indeed, animals conditioned by red/blue lights greatly reduced latency time in the presence of ORX-A while neither MUS nor BIC alone modified such a parameter, with the exception of ORX-A+MUS being responsible for a moderate decrease of latency time with respect to conditioned fish treated with a saline solution. Conversely, ORX-A+MUS/BIC seemed to interfere with ORX-A actions as shown by their very great increase in latency time. Moreover, T-maze results appeared to be strengthened by evident ORXR transcriptional variations especially by the very great mRNA densities detected in some telencephalic regions of animals treated with ORX-A. Of all telencephalic regions Dl, considered homologous to the mammalian hippocampus, proved to be a major target for ORX-A effects. Overall, these data suggest that it is mainly the ORXergic system that promotes feeding behaviors via reward pathways in teleost fish as in mammals. Surprisingly, β GABA(A)R drugs did not modify such behaviors when given alone while the inhibitory effect on cognitive/reward processes was evoked when given together with ORX-A, suggesting that more than β subunits other GABA(A)R subunits could be promoting mnemonically guided motor behaviors.

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Eating / drug effects
  • Eating / physiology
  • Feeding Behavior / drug effects
  • Feeding Behavior / physiology*
  • GABA-A Receptor Agonists / pharmacology
  • GABA-A Receptor Antagonists / pharmacology
  • Goldfish / metabolism
  • Goldfish / physiology*
  • In Situ Hybridization
  • Intracellular Signaling Peptides and Proteins / pharmacology
  • Maze Learning / drug effects
  • Maze Learning / physiology
  • Motor Activity / drug effects
  • Motor Activity / physiology*
  • Neuropeptides / pharmacology
  • Orexin Receptors
  • Orexins
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, GABA-A / genetics
  • Receptors, GABA-A / metabolism*
  • Receptors, Neuropeptide / genetics
  • Receptors, Neuropeptide / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Reward
  • Time Factors

Substances

  • GABA-A Receptor Agonists
  • GABA-A Receptor Antagonists
  • Intracellular Signaling Peptides and Proteins
  • Neuropeptides
  • Orexin Receptors
  • Orexins
  • Receptors, G-Protein-Coupled
  • Receptors, GABA-A
  • Receptors, Neuropeptide