In vivo flow speed measurement of capillaries by photoacoustic correlation spectroscopy

Opt Lett. 2011 Oct 15;36(20):4017-9. doi: 10.1364/OL.36.004017.

Abstract

We recently proposed photoacoustic correlation spectroscopy (PACS) and demonstrated a proof-of-concept experiment. Here we use the technique for in vivo flow speed measurement in capillaries in a chick embryo model. The photoacoustic microscopy system is used to render high spatial resolution and high sensitivity, enabling sufficient signals from single red blood cells. The probe beam size is calibrated by a blood-mimicking phantom. The results indicate the feasibility of using PACS to study flow speeds in capillaries.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blood Circulation / physiology*
  • Blood Flow Velocity
  • Calibration
  • Capillaries / physiology*
  • Chick Embryo
  • Erythrocytes / physiology
  • Phantoms, Imaging
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods*