Rapid release of endothelin and ATP from isolated aortic endothelial cells exposed to increased flow

Biochem Biophys Res Commun. 1990 Jul 31;170(2):649-56. doi: 10.1016/0006-291x(90)92141-l.


Freshly harvested rabbit aortic endothelial cells on filters were exposed to two 3 min periods of a sixfold increase in flow rate of the perfusion buffer. This led to an increase in the levels of endothelin and ATP in the perfusate; arginine vasopressin remained at the basal level. Less ATP was released on the second exposure to high flow; however, endothelin release was not diminished. Using immunohistochemical techniques, endothelin and arginine vasopressin were localised in the same population of endothelial cells; endothelin and vasopressin were present in approximately 90% and 70% of endothelial cells, respectively, which suggests that there is some co-localisation. This is the first time that a stimulation has been shown to produce rapid release of endothelin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Aorta / metabolism
  • Arginine Vasopressin / metabolism
  • Cells, Cultured
  • Endothelins
  • Endothelium, Vascular / metabolism*
  • Male
  • Peptides / metabolism*
  • Perfusion
  • Rabbits


  • Endothelins
  • Peptides
  • Arginine Vasopressin
  • Adenosine Triphosphate