Impulsive pressurization of neuronal cells for traumatic brain injury study

J Vis Exp. 2011 Oct 12;(56):2723. doi: 10.3791/2723.

Abstract

A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injury (TBI). We demonstrate in this video article how blast TBI-relevant impulsive pressurization is applied to the neuronal cells in vitro. This is achieved by using well-controlled pressure pulse created by a specialized Kolsky bar device, with complete pressure history within the cell pressurization chamber recorded. Pressurized neuronal cells are inspected immediately after pressurization, or further incubated to examine the long-term effects of impulsive pressurization on neurite/axonal outgrowth, neuronal gene expression, apoptosis, etc. We observed that impulsive pressurization at about 2 MPa induces distinct neurite loss relative to unpressurized cells. Our technique provides a novel method to investigate the molecular/cellular mechanisms of blast TBI, via impulsive pressurization of brain cells at well-controlled pressure magnitude and duration.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Video-Audio Media

MeSH terms

  • Biomechanical Phenomena
  • Blast Injuries / pathology*
  • Brain Injuries / etiology
  • Brain Injuries / pathology*
  • Cell Line, Tumor
  • Cytological Techniques / instrumentation*
  • Cytological Techniques / methods
  • Humans
  • Neuroblastoma
  • Neurons / pathology*
  • Pressure