Nitric oxide synthase inhibition delays low-frequency stimulation-induced satellite cell activation in rat fast-twitch muscle

Appl Physiol Nutr Metab. 2011 Dec;36(6):996-1000. doi: 10.1139/h11-091. Epub 2011 Oct 20.

Abstract

This study examined the effect of nitric oxide synthase (NOS) inhibition via N(ω)-nitro-l-arginine methyl ester (l-NAME) administration on low-frequency stimulation-induced satellite cell (SC) activation in rat skeletal muscle. l-NAME only delayed stimulation-induced increases in SC activity. Also, stimulation-induced increases in hepatocyte growth factor (HGF) mRNA and protein expression were only abrogated at the mRNA level in l-NAME-treated animals. Therefore, early stimulation-induced SC activation appears to be NOS-dependent, while continued activation may involve NOS-independent HGF translational control mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Electric Stimulation
  • Enzyme Inhibitors / pharmacology*
  • Gene Expression Regulation / drug effects
  • Hepatocyte Growth Factor / genetics
  • Hepatocyte Growth Factor / metabolism
  • Hindlimb
  • Male
  • Muscle Contraction / drug effects*
  • Muscle Fibers, Fast-Twitch / drug effects*
  • Muscle Fibers, Fast-Twitch / metabolism*
  • Muscle Fibers, Fast-Twitch / ultrastructure
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Physical Endurance
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Satellite Cells, Skeletal Muscle / drug effects*
  • Satellite Cells, Skeletal Muscle / metabolism
  • Satellite Cells, Skeletal Muscle / ultrastructure
  • Tachyphylaxis

Substances

  • Enzyme Inhibitors
  • RNA, Messenger
  • Hepatocyte Growth Factor
  • Nitric Oxide Synthase
  • NG-Nitroarginine Methyl Ester