Abstract
Aberrations in IL-3, GM-CSF and G-CSF induced signaling are frequently reported in acute myeloid leukemia (AML). Herein, we utilized a unique human myeloid leukemic cell line, AML-193, which responds to all three cytokines to analyze the regulation at microRNA level. Using real-time PCR-based miRNA expression profiling, we investigated miRNA signatures regulated by IL-3, GM-CSF and G-CSF for n=704 miRNAs. We discovered that in addition to regulating specific miRNAs, these cytokines also regulate common set of miRNAs, which includes miR-590-5p, miR-219-5p, miR-15b and miR-628-5p. Taken together, we have identified novel candidate miRNAs that may be instructive during leukemic and normal hematopoiesis.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers, Tumor / genetics
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic / drug effects*
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Granulocyte Colony-Stimulating Factor / pharmacology*
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
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Humans
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Interleukin-3 / pharmacology*
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Leukemia, Myeloid, Acute / drug therapy*
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Leukemia, Myeloid, Acute / genetics*
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MicroRNAs / genetics*
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Oligonucleotide Array Sequence Analysis
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Real-Time Polymerase Chain Reaction
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Signal Transduction
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Tumor Cells, Cultured
Substances
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Biomarkers, Tumor
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Interleukin-3
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MIRN15 microRNA, human
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MIRN590 microRNA, human
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MIRN629 microRNA, human
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MicroRNAs
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Granulocyte Colony-Stimulating Factor
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Granulocyte-Macrophage Colony-Stimulating Factor