Isoleucine or valine deprivation stimulates fat loss via increasing energy expenditure and regulating lipid metabolism in WAT

Amino Acids. 2012 Aug;43(2):725-34. doi: 10.1007/s00726-011-1123-8. Epub 2011 Oct 21.


There has been a growing interest in controlling body weight by increasing dietary levels of leucine recently. By contrast, we have focused on studying the effect of deficiency of branched-chain amino acids (BCAAs) leucine on lipid metabolism. We previously have shown that mice fed a leucine-deficient diet for 7 days exhibit significant changes in lipid metabolism as demonstrated by suppressed lipogenesis in the liver and increased fat mobilization in white adipose tissue, the latter of which was found to be caused by increased lipolysis in WAT and uncoupling protein 1 expression in brown adipose tissue. The goal of our current study is to investigate whether the above effects of leucine deficiency can be generalized to the deficiency of other BCAAs including valine and isoleucine. In our current study, we show that valine or isoleucine deficiency has similar effects on reducing fat mass to leucine deprivation, in a similar manner as those observed during leucine deprivation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / metabolism
  • Adipose Tissue, White / enzymology
  • Adipose Tissue, White / metabolism*
  • Adiposity
  • Animals
  • Energy Intake
  • Energy Metabolism
  • Fatty Acid Synthases / genetics
  • Fatty Acid Synthases / metabolism
  • Food, Formulated
  • Gene Expression
  • Ion Channels / genetics
  • Ion Channels / metabolism
  • Isoleucine / deficiency*
  • Lipid Metabolism* / genetics
  • Liver / enzymology
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism
  • Oxygen Consumption
  • Uncoupling Protein 1
  • Valine / deficiency*
  • Weight Loss


  • Ion Channels
  • Mitochondrial Proteins
  • Ucp1 protein, mouse
  • Uncoupling Protein 1
  • Isoleucine
  • Fatty Acid Synthases
  • Valine