Mediterranean diet supplemented with coenzyme Q10 modifies the expression of proinflammatory and endoplasmic reticulum stress-related genes in elderly men and women

J Gerontol A Biol Sci Med Sci. 2012 Jan;67(1):3-10. doi: 10.1093/gerona/glr167. Epub 2011 Oct 20.

Abstract

We have investigated whether the quality of dietary fat and supplementation with coenzyme Q(10) (CoQ) modifies expression of genes related with inflammatory response and endoplasmic reticulum stress in elderly persons. Twenty participants received three diets for 4 weeks each: Mediterranean diet + CoQ (Med + CoQ), Mediterranean diet (Med), and saturated fatty acid-rich diet (SFA). After 12-hour fast, volunteers consumed a breakfast with a fat composition similar to that consumed in each of the diets. Med and Med + CoQ diets produced a lower fasting calreticulin, IL-1b, and JNK-1 gene expression; a lower postprandial p65, IKK-b, MMP-9, IL-1b, JNK-1, sXBP-1, and BiP/Grp78 gene expression; and a higher postprandial IkB-a gene expression compared with the SFA diet. Med + CoQ diet produced a lower postprandial decrease p65 and IKK-b gene expression compared with the other diets. Our results support the anti-inflammatory effect of Med diet and that exogenous CoQ supplementation in synergy with a Med diet modulates the inflammatory response and endoplasmic reticulum stress.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Body Mass Index
  • Calreticulin / biosynthesis
  • Calreticulin / genetics
  • Cross-Over Studies
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics
  • Diet, Mediterranean*
  • Dietary Fats / metabolism
  • Dietary Supplements*
  • Endoplasmic Reticulum Stress / drug effects*
  • Female
  • Gene Expression Regulation / drug effects
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics
  • Humans
  • I-kappa B Kinase / biosynthesis
  • I-kappa B Kinase / genetics
  • Inflammation / drug therapy
  • Inflammation / metabolism*
  • Interleukin-1beta / biosynthesis
  • Interleukin-1beta / genetics
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / metabolism
  • Male
  • Matrix Metalloproteinase 9 / biosynthesis
  • Matrix Metalloproteinase 9 / genetics
  • Mitogen-Activated Protein Kinase 8 / biosynthesis
  • Mitogen-Activated Protein Kinase 8 / genetics
  • Regulatory Factor X Transcription Factors
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Ubiquinone / administration & dosage
  • Ubiquinone / analogs & derivatives*
  • Ubiquinone / blood
  • Ubiquinone / metabolism

Substances

  • Calreticulin
  • DNA-Binding Proteins
  • Dietary Fats
  • Heat-Shock Proteins
  • Interleukin-1beta
  • Regulatory Factor X Transcription Factors
  • Transcription Factors
  • Ubiquinone
  • I-kappa B Kinase
  • Mitogen-Activated Protein Kinase 8
  • Matrix Metalloproteinase 9
  • coenzyme Q10
  • molecular chaperone GRP78