The complete nucleotide sequence of the Pasteurella multocida toxin gene and evidence for a transcriptional repressor, TxaR

Mol Microbiol. 1990 May;4(5):821-30. doi: 10.1111/j.1365-2958.1990.tb00652.x.


The osteolytic toxin of Pasteurella multocida induces bone resorption in vivo and in vitro (Foged et al., 1988; Kimman et al., 1987). In this report the toxin-encoding toxA gene is sequenced, and the deduced primary structure of the toxin shows a protein of 1285 amino acids, containing a striking homology to a metal-binding motif. Evidence that expression of the toxA gene is repressed at a transcriptional level in Escherichia coli is presented. Repression could be abolished either by deletion of a region upstream of toxA, or by a putative frame-shift mutation in the same region. The repressor protein encoded within this region was efficient in trans, and was named TxaR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins*
  • Bacterial Toxins / biosynthesis
  • Bacterial Toxins / genetics*
  • Base Sequence
  • Escherichia coli / genetics
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Pasteurella / genetics*
  • Promoter Regions, Genetic
  • RNA, Messenger / analysis
  • Repressor Proteins / biosynthesis
  • Repressor Proteins / genetics*
  • Restriction Mapping
  • Transcription Factors / genetics*
  • Transcription, Genetic


  • Bacterial Proteins
  • Bacterial Toxins
  • Pasteurella multocida toxin
  • RNA, Messenger
  • Repressor Proteins
  • Transcription Factors

Associated data

  • GENBANK/X51512