GATA3 and a dominant regulatory gene expression profile discriminate operational tolerance in human transplantation

Clin Immunol. 2012 Feb;142(2):117-26. doi: 10.1016/j.clim.2011.08.015. Epub 2011 Sep 16.


Some organ-transplanted patients achieve a state of "operational tolerance" (OT) in which graft function is maintained after the complete withdrawal of immunosuppressive drugs. We used a gene panel of regulatory/inflammatory molecules (FOXP3, GATA3, IL10, TGFB1, TGFBR1/ TBX21, TNF and IFNG) to investigate the gene expression profile in peripheral blood mononuclear cells of renal-transplanted individuals experiencing OT compared to transplanted individuals not displaying OT and healthy individuals (HI). OT subjects showed a predominant regulatory (REG) profile with higher gene expression of GATA3, FOXP3, TGFB1 and TGFB receptor 1 compared to the other groups. This predominant REG gene expression profile displayed stability over time. The significant GATA3 gene and protein expressions in OT individuals suggest that a Th2 deviation may be a relevant pathway to OT. Moreover, the capacity of the REG/INFLAMMA gene panel to discriminate OT by peripheral blood analysis indicates that this state has systemic repercussions.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Female
  • Forkhead Transcription Factors / blood
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / metabolism
  • GATA3 Transcription Factor* / blood
  • GATA3 Transcription Factor* / genetics
  • GATA3 Transcription Factor* / metabolism
  • Gene Expression Profiling
  • Graft Survival / immunology
  • Humans
  • Immunosuppressive Agents / blood
  • Immunosuppressive Agents / metabolism*
  • Kidney Transplantation / immunology*
  • Leukocytes, Mononuclear / physiology*
  • Male
  • Middle Aged
  • Receptors, Transforming Growth Factor beta / blood
  • Receptors, Transforming Growth Factor beta / genetics
  • Receptors, Transforming Growth Factor beta / metabolism
  • Retrospective Studies
  • Th2 Cells / metabolism
  • Transforming Growth Factor beta1 / blood
  • Transforming Growth Factor beta1 / genetics
  • Transforming Growth Factor beta1 / metabolism
  • Transplantation Tolerance* / genetics
  • Transplantation Tolerance* / immunology


  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Immunosuppressive Agents
  • Receptors, Transforming Growth Factor beta
  • TGFB1 protein, human
  • Transforming Growth Factor beta1