Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases

Virology. 1990 Sep;178(1):340-3. doi: 10.1016/0042-6822(90)90417-p.


Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophages / enzymology
  • Bacteriophages / genetics
  • Base Sequence
  • DNA, Viral / analysis
  • Escherichia coli / genetics
  • Plasmids
  • RNA Nucleotidyltransferases / genetics*
  • RNA Viruses / enzymology
  • RNA Viruses / genetics*
  • RNA, Viral / biosynthesis*
  • RNA-Dependent RNA Polymerase / genetics*
  • Transcription, Genetic


  • DNA, Viral
  • Qbeta ribonucleic acid
  • RNA, Viral
  • RNA Nucleotidyltransferases
  • RNA-Dependent RNA Polymerase