From SOMAmer-based biomarker discovery to diagnostic and clinical applications: a SOMAmer-based, streamlined multiplex proteomic assay

PLoS One. 2011;6(10):e26332. doi: 10.1371/journal.pone.0026332. Epub 2011 Oct 17.


Recently, we reported a SOMAmer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust, sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated. We demonstrate that quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our platform, further expanding the breadth of proteomic applications for a wide user community.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Automation
  • Biological Assay / methods*
  • Biomarkers / analysis*
  • Case-Control Studies
  • Diagnostic Techniques and Procedures*
  • Humans
  • Limit of Detection
  • Nucleic Acids / metabolism
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism*
  • Proteomics / methods*
  • Reproducibility of Results
  • Titrimetry


  • Biomarkers
  • Nucleic Acids
  • Oligonucleotides