In vivo two-photon microscopy to 1.6-mm depth in mouse cortex

J Biomed Opt. 2011 Oct;16(10):106014. doi: 10.1117/1.3646209.


Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.6 mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cerebral Cortex / anatomy & histology*
  • Cerebral Cortex / blood supply*
  • Cerebrovascular Circulation
  • Fluorescent Dyes / administration & dosage
  • Imaging, Three-Dimensional / methods
  • Male
  • Mice
  • Microscopy, Fluorescence, Multiphoton / methods*
  • Optical Phenomena


  • Fluorescent Dyes