Characterization of marine X-family DNA polymerases and comparative analysis of base excision repair proteins

Biochem Biophys Res Commun. 2011 Nov 11;415(1):193-9. doi: 10.1016/j.bbrc.2011.10.058. Epub 2011 Oct 18.


While mammalian DNA polymerase β (Pol β), which is a member of the Pol X family, play important roles in base excision repair (BER) that efficiently removes DNA base lesions arising from both endogenous and exogenous agents, this protein has been found only a subset of animals. To understand natural evolution of this enzyme, we isolated and characterized Pol β from jellyfish Aurelia sp.1. (AsPol β). Despite of phylogenetic distance and environmental differences between jellyfish and mammals, in vitro assays showed biochemical characteristics of AsPol β were very similar to those of a mammalian counterpart. We also searched two other homologs of mammalian genes that were involved in short patch (sp) BER in the nucleotide sequence database, and found that both of these homologs were encoded in the genomes of a lineage from Cnidarians through mammals and Arthropods. This study suggests that a DNA repair mechanism resembling mammalian sp-BER may be largely limited to a subset of animals. On the basis of our findings and previous reports, we discuss possible evolutional model of Pol β and the other members of the Pol X family.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • DNA Ligase ATP
  • DNA Ligases / chemistry
  • DNA Ligases / genetics
  • DNA Ligases / metabolism
  • DNA Polymerase beta / chemistry
  • DNA Polymerase beta / classification
  • DNA Polymerase beta / genetics
  • DNA Polymerase beta / metabolism*
  • DNA Repair*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Evolution, Molecular
  • Molecular Sequence Data
  • Phylogeny
  • Poly-ADP-Ribose Binding Proteins
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Scyphozoa / enzymology*
  • X-ray Repair Cross Complementing Protein 1
  • Xenopus Proteins


  • DNA-Binding Proteins
  • Poly-ADP-Ribose Binding Proteins
  • Recombinant Proteins
  • X-ray Repair Cross Complementing Protein 1
  • Xenopus Proteins
  • DNA Polymerase beta
  • DNA Ligases
  • DNA Ligase ATP
  • DNA ligase III alpha protein, Xenopus