Comprehensive transcriptome analysis of the periodontopathogenic bacterium Porphyromonas gingivalis W83

J Bacteriol. 2012 Jan;194(1):100-14. doi: 10.1128/JB.06385-11. Epub 2011 Oct 28.

Abstract

High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the strand-specific transcription profiles generated covered the entire genome, including both protein coding and noncoding regions. The transcription profiles revealed various operon structures, 5'- and 3'-end untranslated regions (UTRs), differential expression patterns, and many novel, not-yet-annotated transcripts within intergenic and antisense regions. Further transcriptome analysis identified the majority of the genes as being expressed within operons and most 5' and 3' ends to be protruding UTRs, of which several 3' UTRs were extended to overlap genes carried on the opposite/antisense strand. Extensive antisense RNAs were detected opposite most insertion sequence (IS) elements. Pairwise comparative analyses were also performed among transcriptome profiles of the three culture conditions, and differentially expressed genes and metabolic pathways were identified. With the growing realization that noncoding RNAs play important biological functions, the discovery of novel RNAs and the comprehensive transcriptome profiles compiled in this study may provide a foundation to further understand the gene regulation and virulence mechanisms in P. gingivalis. The transcriptome profiles can be viewed at and downloaded from the Microbial Transcriptome Database website, http://bioinformatics.forsyth.org/mtd.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • Gene Expression Regulation, Bacterial / physiology*
  • Genome, Bacterial
  • Operon
  • Porphyromonas gingivalis / genetics
  • Porphyromonas gingivalis / metabolism*
  • Protein Array Analysis
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • Transcription, Genetic
  • Transcriptome / genetics*

Substances

  • Bacterial Proteins
  • RNA, Bacterial

Associated data

  • GEO/GSE30452