Enhanced Y1H assays for Arabidopsis

Nat Methods. 2011 Oct 30;8(12):1053-5. doi: 10.1038/nmeth.1750.


We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Gene Expression Regulation, Plant
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Promoter Regions, Genetic / genetics*
  • Reproducibility of Results
  • Transcription Factors / metabolism*
  • Two-Hybrid System Techniques*


  • Transcription Factors