Nestin- and doublecortin-positive cells reside in adult spinal cord meninges and participate in injury-induced parenchymal reaction

Stem Cells. 2011 Dec;29(12):2062-76. doi: 10.1002/stem.766.

Abstract

Adult spinal cord has little regenerative potential, thus limiting patient recovery following injury. In this study, we describe a new population of cells resident in the adult rat spinal cord meninges that express the neural stem/precursor markers nestin and doublecortin. Furthermore, from dissociated meningeal tissue a neural stem cell population was cultured in vitro and subsequently shown to differentiate into functional neurons or mature oligodendrocytes. Proliferation rate and number of nestin- and doublecortin-positive cells increased in vivo in meninges following spinal cord injury. By using a lentivirus-labeling approach, we show that meningeal cells, including nestin- and doublecortin-positive cells, migrate in the spinal cord parenchyma and contribute to the glial scar formation. Our data emphasize the multiple roles of meninges in the reaction of the parenchyma to trauma and indicate for the first time that spinal cord meninges are potential niches harboring stem/precursor cells that can be activated by injury. Meninges may be considered as a new source of adult stem/precursor cells to be further tested for use in regenerative medicine applied to neurological disorders, including repair from spinal cord injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult Stem Cells / cytology
  • Adult Stem Cells / metabolism
  • Adult Stem Cells / physiology
  • Animals
  • Cell Differentiation
  • Cell Movement
  • Cell Proliferation
  • Electrophysiologic Techniques, Cardiac
  • Gene Expression Profiling
  • Intermediate Filament Proteins / genetics
  • Intermediate Filament Proteins / metabolism*
  • Laminectomy
  • Lentivirus / genetics
  • Lentivirus / metabolism
  • Meninges / cytology
  • Meninges / metabolism*
  • Meninges / physiology
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Nestin
  • Neural Stem Cells / cytology
  • Neural Stem Cells / physiology
  • Neurogenesis
  • Neuropeptides / genetics
  • Neuropeptides / metabolism*
  • Oligodendroglia / cytology
  • Oligodendroglia / metabolism
  • Oligodendroglia / physiology
  • Patch-Clamp Techniques
  • Rats
  • Rats, Sprague-Dawley
  • Regenerative Medicine
  • Spinal Cord Injuries / therapy*
  • Stem Cell Niche

Substances

  • Intermediate Filament Proteins
  • Microtubule-Associated Proteins
  • Nerve Tissue Proteins
  • Nes protein, rat
  • Nestin
  • Neuropeptides
  • doublecortin protein