Quantification of hydroxyprolyl-glycine (Hyp-Gly) in human blood after ingestion of collagen hydrolysate

J Biosci Bioeng. 2012 Feb;113(2):202-3. doi: 10.1016/j.jbiosc.2011.09.024. Epub 2011 Oct 29.


Plasma levels of prolyl-hydroxyproline (Pro-Hyp) and hydroxyprolyl-glycine (Hyp-Gly) in healthy volunteers (n=5) after ingestion of collagen hydrolysate were estimated by liquid chromatography-tandem mass spectrometry. The ratio of Hyp-Gly to Pro-Hyp was distributed in the range of 0.063-0.221. This is a first report for quantification of food-derived Hyp-Gly in human plasma.

MeSH terms

  • Adult
  • Collagen / administration & dosage*
  • Collagen / metabolism
  • Dipeptides / blood*
  • Eating
  • Humans
  • Hydrolysis
  • Male
  • Tandem Mass Spectrometry


  • Dipeptides
  • hydroxyprolyl-glycine
  • prolyl-4-hydroxyproline
  • Collagen