Abstract
Progesterone and estrogens play an important role in the control of growth, differentiation and function of mammary epithelial cells. Their mechanism of action can be studied in human metastatic breast cancer cell lines (MCF7, T47D, ZR75-1...) that contain progesterone and estrogen receptors. We used this system to try to define progestin-regulated human genes which would permit to study progestin-regulation of gene expression in cell culture and to develop clinical markers of progestin-responsiveness. This paper summarizes our investigation of the progestin-regulated 250K protein, recently identified as human fatty acid synthetase (FAS).
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Breast / enzymology
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Breast Diseases / enzymology
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Breast Neoplasms / analysis
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Breast Neoplasms / enzymology*
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Breast Neoplasms / pathology
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Endometrium / enzymology
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Enzyme Induction / drug effects
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Epithelium / drug effects
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Epithelium / enzymology
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Fatty Acid Synthases / biosynthesis*
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Fatty Acid Synthases / genetics
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Female
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Gene Expression Regulation, Neoplastic / drug effects*
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Humans
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Neoplasms, Hormone-Dependent / analysis
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Neoplasms, Hormone-Dependent / enzymology*
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Neoplasms, Hormone-Dependent / pathology
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Norpregnadienes / pharmacology*
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Promegestone / pharmacology*
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Receptors, Estrogen / analysis
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Receptors, Progesterone / analysis
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / enzymology
Substances
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Neoplasm Proteins
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Norpregnadienes
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Receptors, Estrogen
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Receptors, Progesterone
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Promegestone
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Fatty Acid Synthases