Impairment of macrophage colony-stimulating factor production and lack of resident bone marrow macrophages in the osteopetrotic op/op mouse

J Bone Miner Res. 1990 Jul;5(7):781-9. doi: 10.1002/jbmr.5650050716.


Mouse calvaria-derived osteoblastlike cells have been shown to produce macrophage colony-stimulating factor (M-CSF). This factor may be involved in osteoclastogenesis and thus in bone resorption. In the present study we investigated whether the production of M-CSF was altered in the osteopetrotic mouse mutant strain op/op, characterized by a decrease in osteoclast number and an impairment of bone resorption. Whole calvariae and cells, as well as skin and lung fibroblasts, of the op/op mouse were found to produce no measurable M-CSF, in contrast to tissue and cells derived from normal littermates. M-CSF was identified by colony assay in semisolid media and by inhibition of the biologic activity with antiserum against M-CSF. Furthermore, the number of resident macrophages, identified by F4/80 antigen (F4/80 Ag) immunohistochemistry, was drastically decreased in bone and bone marrow of the op/op mouse, but in skin these cells were normal in number and morphology. These findings suggest that both M-CSF and resident macrophages play a role in the mechanism of bone resorption. The op/op mouse appears to be a valuable model to further investigate such a hypothesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Bone Marrow Cells*
  • Cells, Cultured
  • Colony-Stimulating Factors / antagonists & inhibitors
  • Colony-Stimulating Factors / biosynthesis*
  • Colony-Stimulating Factors / metabolism
  • Female
  • Fibroblasts / metabolism
  • Lung / cytology
  • Lung / metabolism
  • Macrophage Colony-Stimulating Factor
  • Macrophages / pathology*
  • Male
  • Mice
  • Mice, Mutant Strains
  • Neutralization Tests
  • Osteopetrosis / genetics
  • Osteopetrosis / metabolism*
  • Osteopetrosis / pathology
  • Skin / cytology
  • Skin / metabolism
  • Skull / cytology
  • Skull / metabolism


  • Antibodies, Monoclonal
  • Colony-Stimulating Factors
  • Macrophage Colony-Stimulating Factor