Association of NCF2, IKZF1, IRF8, IFIH1, and TYK2 with systemic lupus erythematosus

PLoS Genet. 2011 Oct;7(10):e1002341. doi: 10.1371/journal.pgen.1002341. Epub 2011 Oct 27.


Systemic lupus erythematosus (SLE) is a complex trait characterised by the production of a range of auto-antibodies and a diverse set of clinical phenotypes. Currently, ~8% of the genetic contribution to SLE in Europeans is known, following publication of several moderate-sized genome-wide (GW) association studies, which identified loci with a strong effect (OR>1.3). In order to identify additional genes contributing to SLE susceptibility, we conducted a replication study in a UK dataset (870 cases, 5,551 controls) of 23 variants that showed moderate-risk for lupus in previous studies. Association analysis in the UK dataset and subsequent meta-analysis with the published data identified five SLE susceptibility genes reaching genome-wide levels of significance (P(comb)<5×10(-8)): NCF2 (P(comb) = 2.87×10(-11)), IKZF1 (P(comb) = 2.33×10(-9)), IRF8 (P(comb) = 1.24×10(-8)), IFIH1 (P(comb) = 1.63×10(-8)), and TYK2 (P(comb) = 3.88×10(-8)). Each of the five new loci identified here can be mapped into interferon signalling pathways, which are known to play a key role in the pathogenesis of SLE. These results increase the number of established susceptibility genes for lupus to ~30 and validate the importance of using large datasets to confirm associations of loci which moderately increase the risk for disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Cohort Studies
  • DEAD-box RNA Helicases / genetics*
  • Genetic Predisposition to Disease
  • Genome-Wide Association Study
  • Humans
  • Ikaros Transcription Factor / genetics*
  • Interferon Regulatory Factors / genetics*
  • Interferon-Induced Helicase, IFIH1
  • Interferons / genetics
  • Interferons / metabolism*
  • Lupus Erythematosus, Systemic / genetics*
  • NADPH Oxidases / genetics*
  • Polymorphism, Single Nucleotide
  • Signal Transduction
  • TYK2 Kinase / genetics*


  • IKZF1 protein, human
  • Interferon Regulatory Factors
  • interferon regulatory factor-8
  • Ikaros Transcription Factor
  • Interferons
  • NADPH Oxidases
  • NCF2 protein, human
  • TYK2 Kinase
  • TYK2 protein, human
  • IFIH1 protein, human
  • DEAD-box RNA Helicases
  • Interferon-Induced Helicase, IFIH1