Differential expression of CD300a/c on human TH1 and TH17 cells

BMC Immunol. 2011 Nov 2;12:62. doi: 10.1186/1471-2172-12-62.

Abstract

Background: Human memory CD4+ T cells can be either CD300a/c+ or CD300a/c- and subsequent analyses showed that CD4+ effector memory T (T(EM)) cells are mostly CD300a/c+, whereas CD4+ central memory T (T(CM)) cells have similar frequencies of CD300a/c+ and CD300a/c- cells.

Results: Extensive phenotypical and functional characterization showed that in both T(CM) and T(EM) cells, the CD300a/c+ subset contained a higher number of TH1 (IFN-γ producing) cells. Alternatively, TH17 (IL-17a producing) cells tend to be CD300a/c-, especially in the T(EM) subset. Further characterization of the IL-17a+ cells showed that cells that produce only this cytokine are mostly CD300a/c-, while cells that produce IL-17a in combination with other cytokines, especially IFN-γ, are mostly CD300a/c+, indicating that the expression of this receptor is associated with cells that produce IFN-γ. Co-ligation of the TCR and CD300a/c in CD4+ T cells inhibited Ca2+ mobilization evoked by TCR ligation alone and modulated IFN-γ production on TH1 polarized cells.

Conclusion: We conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells and that their ligation is capable of modulating TCR mediated signals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / genetics
  • Antigens, CD / immunology
  • Antigens, CD / metabolism*
  • Antigens, Surface / genetics
  • Antigens, Surface / immunology
  • Antigens, Surface / metabolism*
  • Calcium Signaling / immunology
  • Cell Differentiation / immunology
  • Cell Lineage / immunology
  • Cells, Cultured
  • Gene Expression Regulation / immunology
  • Humans
  • Immunologic Memory
  • Immunophenotyping
  • Interferon-gamma / metabolism
  • Interleukin-17 / metabolism
  • Lymphocyte Activation
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / metabolism*
  • Receptor Cross-Talk / immunology
  • Receptors, Antigen, T-Cell, alpha-beta / immunology
  • Receptors, Antigen, T-Cell, alpha-beta / metabolism
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / immunology
  • Receptors, Immunologic / metabolism*
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism*
  • T-Lymphocyte Subsets / pathology
  • Th1 Cells / immunology
  • Th1 Cells / metabolism*
  • Th1 Cells / pathology
  • Th17 Cells / immunology
  • Th17 Cells / metabolism*
  • Th17 Cells / pathology

Substances

  • Antigens, CD
  • Antigens, Surface
  • CD300A protein, human
  • CD300C protein, human
  • Interleukin-17
  • Membrane Glycoproteins
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Immunologic
  • Interferon-gamma