Molecular identification of the sperm selection involved porcine sperm binding glycoprotein (SBG) as deleted in malignant brain tumors 1 (DMBT1)

Biochimie. 2012 Jan;94(1):263-7. doi: 10.1016/j.biochi.2011.10.008. Epub 2011 Oct 25.

Abstract

Porcine sperm binding glycoprotein (SBG) is involved in sperm-oviduct interaction. Here we use mass spectrometry to identify SBG, finding peptides corresponding to deleted in malignant brain tumors 1 (DMBT1), at scavenger receptor cysteine-rich (SRCR) and CUB domains. RT-PCR allowed the cloning of unique sequences, belonging to porcine DMBT1. Western blot and immunofluorescence of oviductal tissues using anti-SBG and anti-hDMBT1 antibodies showed identical results. The biochemical characteristics of both proteins are coincident. We conclude that porcine SBG is an oviductal form of DMBT1, and thus assign this protein a novel location and function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • DNA, Complementary
  • Fluorescent Antibody Technique
  • Glycoproteins / chemistry
  • Glycoproteins / metabolism*
  • Male
  • Molecular Sequence Data
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Seminal Plasma Proteins / chemistry
  • Seminal Plasma Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Spermatozoa / metabolism*
  • Swine
  • Tandem Mass Spectrometry

Substances

  • DNA, Complementary
  • Glycoproteins
  • Receptors, Cell Surface
  • Seminal Plasma Proteins
  • spermadhesin