Expression of α-crystallin in the retina of human sympathetic ophthalmia

Mol Med Rep. 2012 Feb;5(2):395-9. doi: 10.3892/mmr.2011.653. Epub 2011 Nov 1.


Sympathetic ophthalmia (SO) is a bilateral, granulomatous, intraocular inflammation that occurs following a penetrating injury to one eye, and has the potential to cause blindness of both eyes. The aim of this study was to examine the expression of α-crystallin and to detect apoptotic cells in the retina of human eyes with SO. Five globes, including three with SO and two age-matched normal appearing retinae, were examined. Formalin-fixed, paraffin-embedded tissue sections were submitted to hematoxylin and eosin staining and immuno-histochemistry with anti-αA and αB-crystallin antibodies. Apoptotic cells were detected using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method, and double-staining immunohistochemistry was conducted together with the TUNEL reaction. In normal-appearing retina, αA-crystallin immunoreactivity was predominantly detected in the cytoplasm of photoreceptors, where αB-crystallin was less marked. In SO globes, granulomatous inflammation was noted in the choroid, whereas the retina and choriocapillaris were preserved. Immunoreactivity for αA-crystallin was detected in the retina, as well as in the cytoplasm and inner/outer photoreceptor segments. By contrast, αB-crystallin was weakly noted in the SO retina. Double-staining immuno-histochemistry revealed no TUNEL-positive photoreceptors in the retina displaying high immunoreactivity for αA-crystallin, but photoreceptor apoptosis was noted where expression of αA-crystallin was relatively low. The present study demonstrated that αA-crystallin was up-regulated in the cytoplasm of photoreceptors in the SO retina. This may play a protective role in the suppression of photoreceptor apoptosis associated with intraocular inflammation.

Keywords: α-crystallin; apoptosis; retina; sympathetic ophthalmia.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Apoptosis
  • Gene Expression Regulation*
  • Humans
  • Immunohistochemistry
  • In Situ Nick-End Labeling
  • Ophthalmia, Sympathetic / metabolism*
  • Ophthalmia, Sympathetic / pathology
  • Photoreceptor Cells / metabolism
  • Retina / metabolism*
  • alpha-Crystallin A Chain / metabolism
  • alpha-Crystallin B Chain / metabolism
  • alpha-Crystallins / genetics
  • alpha-Crystallins / metabolism*


  • alpha-Crystallin A Chain
  • alpha-Crystallin B Chain
  • alpha-Crystallins