Sox9 is upstream of microRNA-140 in cartilage

Appl Biochem Biotechnol. 2012 Jan;166(1):64-71. doi: 10.1007/s12010-011-9404-y. Epub 2011 Nov 4.

Abstract

MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a-/-) and sox9b mutant (sox9b-/-) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Binding Sites
  • Cartilage / growth & development*
  • Cartilage / metabolism
  • Chondrocytes / metabolism
  • Chondrogenesis / genetics
  • Embryonic Development / genetics
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • Phylogeny
  • Protein Binding
  • RNA, Small Interfering / genetics
  • SOX9 Transcription Factor / genetics*
  • SOX9 Transcription Factor / metabolism*
  • Ubiquitin-Protein Ligases / genetics
  • Zebrafish / genetics
  • Zebrafish / growth & development*

Substances

  • MicroRNAs
  • Mirn140 microRNA, human
  • RNA, Small Interfering
  • SOX9 Transcription Factor
  • SOX9 protein, human
  • WWP2 protein, human
  • Ubiquitin-Protein Ligases