Molecular cloning and characterization of a novel human papillomavirus, HPV 126, isolated from a flat wart-like lesion with intracytoplasmic inclusion bodies and a peculiar distribution of Ki-67 and p53

Virology. 2012 Jan 5;422(1):99-104. doi: 10.1016/j.virol.2011.10.011. Epub 2011 Nov 5.


Infection with certain human papillomavirus types induces warts with specific macroscopic and microscopic features. We observed multiple flat wart-like lesions on the chest, neck and extremities of an adult T-cell leukemia patient. Histologically, atypical intracytoplasmic inclusion bodies currently known to be pathognomonic for genus gamma or mu papillomaviruses were disclosed in some cells of the epidermis showing histological features compatible with flat warts. In the present study, a novel human papillomavirus was identified and its whole genome, 7326 bp in length, was cloned and characterized. Phylogenetic analysis showed the virus designated as HPV126 to be a novel type of genus gamma papillomavirus. Strikingly, Ki-67 and p53 expression was found to be increased in all layers of the epidermis except for horny layer, contrasting to expression restricted to the basal and lower spinous layers in ordinary flat warts.

MeSH terms

  • Base Sequence
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • Gammapapillomavirus / genetics*
  • Gammapapillomavirus / isolation & purification*
  • Humans
  • Inclusion Bodies, Viral
  • Japan
  • Ki-67 Antigen / analysis
  • Leukemia, T-Cell / complications
  • Male
  • Middle Aged
  • Sequence Analysis, DNA
  • Skin / virology
  • Tumor Suppressor Protein p53 / analysis
  • Warts / pathology
  • Warts / virology*


  • DNA, Viral
  • Ki-67 Antigen
  • Tumor Suppressor Protein p53