Electrochemical control of growth factor presentation to steer neural stem cell differentiation

Angew Chem Int Ed Engl. 2011 Dec 23;50(52):12529-33. doi: 10.1002/anie.201103728. Epub 2011 Nov 4.

Abstract

Let it grow: The conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was synthesized with heparin as the counterion to form a cell culture substrate. The surface of PEDOT:heparin in the neutral state associated biologically active growth factors. Electrochemical in situ oxidation of PEDOT during live cell culture decreased the bioavailability of the growth factor and created an exact onset of neural stem cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bridged Bicyclo Compounds, Heterocyclic / chemistry
  • Cell Differentiation
  • Cell Proliferation
  • Electrochemistry
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism
  • Fibroblast Growth Factor 2 / chemistry*
  • Fibroblast Growth Factor 2 / metabolism
  • Heparin / chemistry
  • Polymers / chemistry

Substances

  • Bridged Bicyclo Compounds, Heterocyclic
  • Polymers
  • poly(3,4-ethylene dioxythiophene)
  • Fibroblast Growth Factor 2
  • Heparin