Prebiotic effects and intestinal fermentation of cereal arabinoxylans and arabinoxylan oligosaccharides in rats depend strongly on their structural properties and joint presence

Mol Nutr Food Res. 2011 Dec;55(12):1862-74. doi: 10.1002/mnfr.201100377. Epub 2011 Nov 7.

Abstract

Scope: Cereal arabinoxylan (AX) is one of the main dietary fibers in a balanced human diet. To gain insight into the importance of structural features of AX for their prebiotic potential and intestinal fermentation properties, a rat trial was performed.

Methods and results: A water unextractable AX-rich preparation (WU-AX, 40% purity), water extractable AX (WE-AX, 81% purity), AX oligosaccharides (AXOS, 79% purity) and combinations thereof were included in a standardized diet at a 5% AX level. WU-AX was only partially fermented in the ceco-colon and increased the level of butyrate and of butyrate producing Roseburia/E. rectale spp. Extensive fermentation of WE-AX and/or AXOS reduced the pH, suppressed relevant markers of the proteolytic breakdown and induced a selective bifidogenic response. Compared with WE-AX, AXOS showed a slightly less pronounced effect in the colon as its fermentation was virtually complete in the cecum. Combining WU-AX and AXOS caused a striking synergistic increase in cecal butyrate levels. WU-AX, WE-AX and AXOS together combined a selective bifidogenic effect in the colon with elevated butyrate levels, a reduced pH and suppressed proteolytic metabolites.

Conclusion: The prebiotic potential and fermentation characteristics of cereal AX depend strongly on their structural properties and joint presence.

MeSH terms

  • Animals
  • Bifidobacterium / drug effects
  • Butyrates / metabolism
  • Diet
  • Dietary Fiber / administration & dosage
  • Edible Grain / chemistry*
  • Fermentation
  • Intestinal Mucosa / metabolism*
  • Intestines / drug effects*
  • Lactobacillaceae / drug effects
  • Male
  • Oligosaccharides / metabolism*
  • Prebiotics*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Xylans / metabolism*

Substances

  • Butyrates
  • Dietary Fiber
  • Oligosaccharides
  • Prebiotics
  • Xylans
  • arabinoxylan