Treatment with probiotics in experimental oral colonization by Candida albicans in murine model (DBA/2)

Oral Dis. 2012 Apr;18(3):260-4. doi: 10.1111/j.1601-0825.2011.01868.x. Epub 2011 Nov 8.


The aim of this study is to evaluate the oral colonization by Candida albicans in experimental murine immunosuppressed DBA/2 and treatment with probiotic bacteria. To achieve these objectives, 152 DBA/2-immunosuppressed mice were orally inoculated with a suspension of C. albicans containing 10(8) viable yeast cells, the animals were treated with nystatin or with the probiotics (Lactobacillus acidophilus and Lactobacillus rhamnosus). Evaluations were performed by Candida count from oral mucosa swabbing. The oral mucosa colonization by C. albicans started at day 1 after inoculation, remained maximal from day 3 until day 7, and then decreased significantly. Probiotics reduced the C. albicans colonization significantly on the oral mucosa in comparison with the untreated animal group. In the group treated with L. rhamnosus, the reduction in yeast colonization was significantly higher compared with that of the group receiving nystatin. Immunosuppressed animal model DBA/2 is a relevant model for experimental Candida oral colonization, and the treatment with probiotics in this model may be an effective alternative to prevent it.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antifungal Agents / therapeutic use
  • Candida / isolation & purification
  • Candidiasis, Oral / drug therapy
  • Candidiasis, Oral / therapy*
  • Colony Count, Microbial
  • Cyclophosphamide / administration & dosage
  • Disease Models, Animal
  • Immunosuppression Therapy*
  • Immunosuppressive Agents / administration & dosage
  • Lacticaseibacillus rhamnosus
  • Lactobacillus acidophilus
  • Male
  • Mice
  • Mice, Inbred DBA
  • Mouth Mucosa / microbiology
  • Nystatin / therapeutic use
  • Palate / microbiology
  • Probiotics / therapeutic use*
  • Random Allocation
  • Tongue / microbiology


  • Antifungal Agents
  • Immunosuppressive Agents
  • Nystatin
  • Cyclophosphamide