Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene

P S Girish; A S R Anjaneyulu; K N Viswas; B M Shivakumar; M Anand; M Patel; B Sharma

doi:10.1016/j.meatsci.2004.12.004

Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene

Meat Sci. 2005 May;70(1):107-12. doi: 10.1016/j.meatsci.2004.12.004.

Authors

P S Girish¹, A S R Anjaneyulu, K N Viswas, B M Shivakumar, M Anand, M Patel, B Sharma

Affiliation

¹ Division of Livestock Products Technology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, India.

PMID: 22063286
DOI: 10.1016/j.meatsci.2004.12.004

Abstract

Adulteration of high quality meat and meat products with their inferior/cheaper counterparts is a problem in the meat industry. The present study investigated the use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial 12S rRNA gene for identification of the origin of meats. PCR-RFLP was applied for species identification of beef, buffalo meat, mutton and chevon. PCR amplification yielded a 456-bp fragment in each of these species. The amplicons were digested with AluI, HhaI, ApoI and BspTI restriction enzymes resulting in a pattern that could identify and differentiate each of the above species. This technique did not yield satisfactory results with meat mixtures/meats. However, consistent results were obtained with both fresh and processed meat samples.